rabbit antiserum directed against the sun domain-containing protein 2 (sun2) Search Results


hic-5  (Becton Dickinson)
90
Becton Dickinson hic-5
a Workflow for mass spectrometric characterisation of FAK-proximal nuclear proteins. b , c Proportions of specific FAK-proximal proteins ( P < 0.05, one-sided Student’s t -test with FDR correction) quantified in the meta-adhesome, including the consensus adhesome ( b ), and the literature-curated adhesome ( c ). Tick marks indicate 20% increments. d Interaction network analysis of FAK-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered according to connectivity (reported interactions or proximal associations inferred from BioID data; edges). e Confocal imaging of SCC cells in the presence or absence of 10 nM LMB, as for Fig. , except magenta is <t>Hic-5.</t> Images are representative of three independent experiments. Scale bars, 20 μm. f Quantification of nuclear Hic-5 signal in nucleus-central z -slices (see e ). Black bars, condition mean (thick bar) ± s.d. (thin bars); grey silhouette, probability density. Data from different independent biological replicates (rep.) are indicated by coloured circles; replicate means are indicated by large circles. Statistical analysis, two-sided Welch’s t -test of replicate means ( n = 54 and 52 cells for control and LMB treatment, respectively, from n = 3 independent biological replicates). g Interaction network analysis of the intersection of specific FAK- and Hic-5-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered as for d . h Total cell lysates from SCC cells (top) and quantification of Hic-5 protein expression (bottom). Protein expression was normalised to GAPDH and expressed relative to SCC FAK-WT. i Total cell lysates from SCC FAK-WT cells transfected with two independent Hic-5 shRNAs (sh1, sh2) or empty-vector control (Ctrl) (top) and quantification of Hic-5 protein expression (bottom, as for h ). j , k RT-qPCR analyses of expression of selected genes identified by nuclear multi-omic analysis (see Fig. ) in SCC cells ( j ) and cells depleted of Hic-5 ( k ). Gene expression was normalised to B2m , binary-logarithm transformed and expressed relative to FAK-WT ( j ) or Ctrl ( k ) cells. For h – k , black bar, median; light grey box, range. Statistical analysis, one-way ANOVA with Tukey’s correction ( n = 3 independent biological replicates). Source data are provided as a Source Data file.
Hic 5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antiserum+directed+against+the+sun+domain-containing+protein+2+(sun2)/hic+5+antibody/pmc09160004-247-96-98
Average 90 stars, based on 1 article reviews
hic-5 - by Bioz Stars, 2026-10
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golgin subfamily member 2 (gm130  (Becton Dickinson)
90
Becton Dickinson golgin subfamily member 2 (gm130
a Workflow for mass spectrometric characterisation of FAK-proximal nuclear proteins. b , c Proportions of specific FAK-proximal proteins ( P < 0.05, one-sided Student’s t -test with FDR correction) quantified in the meta-adhesome, including the consensus adhesome ( b ), and the literature-curated adhesome ( c ). Tick marks indicate 20% increments. d Interaction network analysis of FAK-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered according to connectivity (reported interactions or proximal associations inferred from BioID data; edges). e Confocal imaging of SCC cells in the presence or absence of 10 nM LMB, as for Fig. , except magenta is <t>Hic-5.</t> Images are representative of three independent experiments. Scale bars, 20 μm. f Quantification of nuclear Hic-5 signal in nucleus-central z -slices (see e ). Black bars, condition mean (thick bar) ± s.d. (thin bars); grey silhouette, probability density. Data from different independent biological replicates (rep.) are indicated by coloured circles; replicate means are indicated by large circles. Statistical analysis, two-sided Welch’s t -test of replicate means ( n = 54 and 52 cells for control and LMB treatment, respectively, from n = 3 independent biological replicates). g Interaction network analysis of the intersection of specific FAK- and Hic-5-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered as for d . h Total cell lysates from SCC cells (top) and quantification of Hic-5 protein expression (bottom). Protein expression was normalised to GAPDH and expressed relative to SCC FAK-WT. i Total cell lysates from SCC FAK-WT cells transfected with two independent Hic-5 shRNAs (sh1, sh2) or empty-vector control (Ctrl) (top) and quantification of Hic-5 protein expression (bottom, as for h ). j , k RT-qPCR analyses of expression of selected genes identified by nuclear multi-omic analysis (see Fig. ) in SCC cells ( j ) and cells depleted of Hic-5 ( k ). Gene expression was normalised to B2m , binary-logarithm transformed and expressed relative to FAK-WT ( j ) or Ctrl ( k ) cells. For h – k , black bar, median; light grey box, range. Statistical analysis, one-way ANOVA with Tukey’s correction ( n = 3 independent biological replicates). Source data are provided as a Source Data file.
Golgin Subfamily Member 2 (Gm130, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antiserum+directed+against+the+sun+domain-containing+protein+2+(sun2)/anti+gm130/pmc09160004-247-43-50
Average 90 stars, based on 1 article reviews
golgin subfamily member 2 (gm130 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
afap1  (Becton Dickinson)
90
Becton Dickinson afap1
a Workflow for mass spectrometric characterisation of FAK-proximal nuclear proteins. b , c Proportions of specific FAK-proximal proteins ( P < 0.05, one-sided Student’s t -test with FDR correction) quantified in the meta-adhesome, including the consensus adhesome ( b ), and the literature-curated adhesome ( c ). Tick marks indicate 20% increments. d Interaction network analysis of FAK-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered according to connectivity (reported interactions or proximal associations inferred from BioID data; edges). e Confocal imaging of SCC cells in the presence or absence of 10 nM LMB, as for Fig. , except magenta is <t>Hic-5.</t> Images are representative of three independent experiments. Scale bars, 20 μm. f Quantification of nuclear Hic-5 signal in nucleus-central z -slices (see e ). Black bars, condition mean (thick bar) ± s.d. (thin bars); grey silhouette, probability density. Data from different independent biological replicates (rep.) are indicated by coloured circles; replicate means are indicated by large circles. Statistical analysis, two-sided Welch’s t -test of replicate means ( n = 54 and 52 cells for control and LMB treatment, respectively, from n = 3 independent biological replicates). g Interaction network analysis of the intersection of specific FAK- and Hic-5-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered as for d . h Total cell lysates from SCC cells (top) and quantification of Hic-5 protein expression (bottom). Protein expression was normalised to GAPDH and expressed relative to SCC FAK-WT. i Total cell lysates from SCC FAK-WT cells transfected with two independent Hic-5 shRNAs (sh1, sh2) or empty-vector control (Ctrl) (top) and quantification of Hic-5 protein expression (bottom, as for h ). j , k RT-qPCR analyses of expression of selected genes identified by nuclear multi-omic analysis (see Fig. ) in SCC cells ( j ) and cells depleted of Hic-5 ( k ). Gene expression was normalised to B2m , binary-logarithm transformed and expressed relative to FAK-WT ( j ) or Ctrl ( k ) cells. For h – k , black bar, median; light grey box, range. Statistical analysis, one-way ANOVA with Tukey’s correction ( n = 3 independent biological replicates). Source data are provided as a Source Data file.
Afap1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antiserum+directed+against+the+sun+domain-containing+protein+2+(sun2)/anti+afap/pmc09160004-247-69-71
Average 90 stars, based on 1 article reviews
afap1 - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


a Workflow for mass spectrometric characterisation of FAK-proximal nuclear proteins. b , c Proportions of specific FAK-proximal proteins ( P < 0.05, one-sided Student’s t -test with FDR correction) quantified in the meta-adhesome, including the consensus adhesome ( b ), and the literature-curated adhesome ( c ). Tick marks indicate 20% increments. d Interaction network analysis of FAK-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered according to connectivity (reported interactions or proximal associations inferred from BioID data; edges). e Confocal imaging of SCC cells in the presence or absence of 10 nM LMB, as for Fig. , except magenta is Hic-5. Images are representative of three independent experiments. Scale bars, 20 μm. f Quantification of nuclear Hic-5 signal in nucleus-central z -slices (see e ). Black bars, condition mean (thick bar) ± s.d. (thin bars); grey silhouette, probability density. Data from different independent biological replicates (rep.) are indicated by coloured circles; replicate means are indicated by large circles. Statistical analysis, two-sided Welch’s t -test of replicate means ( n = 54 and 52 cells for control and LMB treatment, respectively, from n = 3 independent biological replicates). g Interaction network analysis of the intersection of specific FAK- and Hic-5-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered as for d . h Total cell lysates from SCC cells (top) and quantification of Hic-5 protein expression (bottom). Protein expression was normalised to GAPDH and expressed relative to SCC FAK-WT. i Total cell lysates from SCC FAK-WT cells transfected with two independent Hic-5 shRNAs (sh1, sh2) or empty-vector control (Ctrl) (top) and quantification of Hic-5 protein expression (bottom, as for h ). j , k RT-qPCR analyses of expression of selected genes identified by nuclear multi-omic analysis (see Fig. ) in SCC cells ( j ) and cells depleted of Hic-5 ( k ). Gene expression was normalised to B2m , binary-logarithm transformed and expressed relative to FAK-WT ( j ) or Ctrl ( k ) cells. For h – k , black bar, median; light grey box, range. Statistical analysis, one-way ANOVA with Tukey’s correction ( n = 3 independent biological replicates). Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Characterisation of a nucleo-adhesome

doi: 10.1038/s41467-022-30556-5

Figure Lengend Snippet: a Workflow for mass spectrometric characterisation of FAK-proximal nuclear proteins. b , c Proportions of specific FAK-proximal proteins ( P < 0.05, one-sided Student’s t -test with FDR correction) quantified in the meta-adhesome, including the consensus adhesome ( b ), and the literature-curated adhesome ( c ). Tick marks indicate 20% increments. d Interaction network analysis of FAK-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered according to connectivity (reported interactions or proximal associations inferred from BioID data; edges). e Confocal imaging of SCC cells in the presence or absence of 10 nM LMB, as for Fig. , except magenta is Hic-5. Images are representative of three independent experiments. Scale bars, 20 μm. f Quantification of nuclear Hic-5 signal in nucleus-central z -slices (see e ). Black bars, condition mean (thick bar) ± s.d. (thin bars); grey silhouette, probability density. Data from different independent biological replicates (rep.) are indicated by coloured circles; replicate means are indicated by large circles. Statistical analysis, two-sided Welch’s t -test of replicate means ( n = 54 and 52 cells for control and LMB treatment, respectively, from n = 3 independent biological replicates). g Interaction network analysis of the intersection of specific FAK- and Hic-5-proximal nuclear proteins present in the consensus or literature-curated adhesomes, clustered as for d . h Total cell lysates from SCC cells (top) and quantification of Hic-5 protein expression (bottom). Protein expression was normalised to GAPDH and expressed relative to SCC FAK-WT. i Total cell lysates from SCC FAK-WT cells transfected with two independent Hic-5 shRNAs (sh1, sh2) or empty-vector control (Ctrl) (top) and quantification of Hic-5 protein expression (bottom, as for h ). j , k RT-qPCR analyses of expression of selected genes identified by nuclear multi-omic analysis (see Fig. ) in SCC cells ( j ) and cells depleted of Hic-5 ( k ). Gene expression was normalised to B2m , binary-logarithm transformed and expressed relative to FAK-WT ( j ) or Ctrl ( k ) cells. For h – k , black bar, median; light grey box, range. Statistical analysis, one-way ANOVA with Tukey’s correction ( n = 3 independent biological replicates). Source data are provided as a Source Data file.

Article Snippet: Membranes were probed with the following primary antibodies (all Cell Signalling Technology, diluted 1:1000 in BSA/TBS-T, unless otherwise stated): α-tubulin (#3873, diluted 1:2000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#2118; #5174), protein disulphide isomerase A4 (ERp72) (#5033), receptor-binding cancer antigen expressed on SiSo cells (RCAS1) (#12290), golgin subfamily A member 2 (GM130) (#610822, BD Biosciences), SUN domain-containing protein 2 (Sun2) (#ab87036, Abcam), lamin-A/C (#4777), poly(ADP-ribose) polymerase 1 (PARP1) (#9532), histone H4 (#2935), AFAP1 (#610200, BD Biosciences), c-Met (#4560), APPL1 (#3858), EEA1 (#2411), cathepsin B (#31718), golgin-97 (#13192), testin (#sc-373913, Santa Cruz Biotechnology), FAK (#3285), histone H3 (#4620), HP1α/β (#2623), Hic-5 (#611164, BD Biosciences).

Techniques: Imaging, Expressing, Transfection, Plasmid Preparation, Quantitative RT-PCR, Transformation Assay